ISBN: | 9781071620588 |
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ISBN: | 1071620584 |
编目源: | UKMGB UKMGB OCLCF OCLCO UKMGB HNK OCLCO MMU |
题名: | Angiogenesis : methods and protocols / Andrew V. Benest. |
出版发行项: | New York : Humana Press, 2022 |
载体形态: | xiv, 429 pages : illustrations (some color) ; 26 cm. |
书目附注: | Includes bibliographical references and index. |
格式化内容附注: | The embryonic mouse hindbrain model to study sprouting angiogenesis in vivo -- A workflow to track and analyze endothelial migration during vascular development in zebrafish embryos using lightsheet microscopy -- Analyzing vessel regression and endothelial apoptosis as a component of angiogenic vessel remodeling -- Whole-mount immunofluorescence protocol for 3D imaging, reconstruction, and quantification of 4th pharyngeal arch artery formation -- Quantifying and characterizing angiogenesis using the postnatal mouse retina -- Characterization of lymphatic vasculature using whole-mount immunostaining of mouse embryonic dorsal skin -- Analyzing lymphatic vessel patterning in adult tissue -- Transmission electron microscopy of endothelium -- Measurement of revascularisation in the hind limb after experimental ischemia in mice -- Imaging blood vessels and lymphatics in mouse trachea wholemounts -- Non-invasive measurement of retinal microvascular permeability during loss of endothelial quiescence -- An ex vivo tissue culture method for discovering cell dynamics involved in stromal vascular fraction vasculogenesis using the mouse mesentery -- Quantitative three-dimensional analysis of the lymphatic vasculature in the postnatal mouse heart -- Quantifying lymphatic vessel density in human tissue samples -- Quantifying vascular remodeling in the mouse spinal cord -- Quantitative methods to assess adipose vasculature -- Quantification of angiogenesis in laser choroidal neovascularization -- A rapid adaptable method for isolation of endothelial cells from human adipose tissue -- Simple gene knockdown in endothelial cells using short interfering rna oligonucleotides -- Transcription factor chromatin immunoprecipitation in endothelial cells -- In vitro co-culture of fibroblast and endothelial cells to assess angiogenesis -- Quantification of leukocyte-endothelial cell interaction during in vitro models of thrombosis at arterial and venous shear rates -- Isolation of cardiac endothelial cells for transcriptomic analysis of the zebrafish and mouse heart -- The evaluation of neovessel angiogenesis behavior at tissue interfaces -- Endothelial cell fibrin gel angiogenesis bead assay -- Live cell imaging and analysis of cancer-cell transmigration through endothelial monolayers -- Differentiation and characterization of human pluripotent stem cell-derived cardiac endothelial cells for in vitro applications -- Finding and verifying enhancers for endothelial-expressed genes -- Endothelial cell RNA-seq data: differential expression and functional enrichment analyses to study phenotypic switching. |