ISBN: | 9781071612040 |
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ISBN: | 1071612042 |
编目源: | NLM NLM UKMGB OCLCF HNK BDX YDX OCLCO |
主款目-统一题名: | Melanoma (Hargadon) |
题名: | Melanoma : methods and protocols / edited by Kristian M. Hargadon. |
出版发行项: | New York : Humana Press, 2021 |
载体形态: | xx, 674 pages : illustrations (some color) ; 26 cm. |
书目附注: | Includes bibliographical references and index. |
格式化内容附注: | Model Systems for the Study of Malignant Melanoma -- Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing -- A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix -- Wound Healing Assay for Melanoma Cell Migration -- A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells -- Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology -- Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry -- Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry -- In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium -- A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells -- An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence -- Evaluating Melanoma Viability and Proliferation in 3D Microenvironments -- Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models -- Enrichment of Melanoma Cancer Stem Cells via Sphere Assays -- Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry -- Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells -- PD-L1 Detection on Circulating Melanoma Cells -- Transcript-Based Detection of Circulating Melanoma Cells -- Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients -- Simultaneous BRAFV600E Protein and DNA Aberration Detection in Circulating Melanoma Cells Using an Integrated Multi-Molecular Sensor -- Single Cell Analysis of BRAFV600E and NRASQ61R Mutation Status in Melanoma Cell Lines as Method Generation for Circulating Melanoma Cells -- A Rapid Exosome Isolation Using Size Exclusion Chromatography (REIUS) Method for Exosome Isolation from Melanoma Cell Lines -- Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients -- An Immunocapture-Based Assay for Detecting Multiple Antigens in Melanoma-Derived Extracellular Vesicles -- Post-Lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients -- Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins -- Detection of Melanoma Cells in Lymphatic Drainage (LD) after Lymph Nodes Dissection via Nested RT-PCR Analysis of Molecular Melanocytic Markers -- A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue -- PET Imaging of Melanoma Using Melanin-Targeted Probe -- Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis -- Salivary Proteomic Analysis of Canine Oral Melanoma by MALDI-TOF Mass Spectrometry and LC-Mass Spectrometry/Mass Spectrometry -- Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers -- Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma -- Assessment of Cell-Free microRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients' Blood -- High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0 -- Immunotyping and Quantification of Melanoma Tumor-Infiltrating Lymphocytes -- Single Cell Gene Expression, Clonality, and Feature Barcoding of Melanoma Tumor Infiltrating Lymphocytes -- Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma -- Multiplex Immunohistochemistry Analysis of Melanoma Tumor Infiltrating Lymphocytes -- Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures -- The Use of Nanoparticles in the Delivery of Nucleic Acids for Melanoma Treatment -- siRNA Delivery to Melanoma Cells with Cationic Niosomes -- Controlled Delivery of Plasmid DNA to Melanoma Tumors by Gene Electrotransfer -- Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma -- Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy. |